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22-01-2008, 06:24 PM
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ha! ha! great night all the talks were bang on but frank and jamie stud out the pictures frank showed of romania were good its a bit rum out there tho people just squating one out in the street whilst everyone carrys on with there day to day lives and when he talked about the guy who owned radutta dealing with fish thieves with an ak47!
 anyway good to catch up with some lads of the forum and a few old pals from the stoke park lake welldone ritchie and Ging on tatton bet you cant remember to much of the night tho ritch funny when frank came back on you were doing some sluring then fella! i to was slightly tipsy but i hadnt won north west angler of the year so had no excuse and finally nice talking to the lads from ultimate carp nutrition they certinally know there bacon n eggs where baits concerned got a bit sick when mat d started talking enzymes went straight over my head and if the truth be known in the end i think mats head was done in aswell!
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getting back on the bank! 
    
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22-01-2008, 06:34 PM
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 Pete
Last edited by netbustercarp; 22-01-2008 at 06:39 PM.
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22-01-2008, 06:39 PM
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yeh it wasnt so much the enzyme more the differnt enzymes used and what they bring to the table regards fish catching best bit of trivia tho i never knew they were used in washing powder to breakdown stains!
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getting back on the bank!
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22-01-2008, 06:41 PM
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used in a lot of things.slippery elm does the trick in your base mixes
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Pete
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22-01-2008, 07:33 PM
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Damn,after 20 years of research and it was right there on the shelf as a digestive aid "SLIPPERY ELM"...........Wish you had told me all those years ago fella
Was great talking to you guys,sorry if it got a little heavy at times but Pete does tend to go off on one when he"s had a few sherberts Great nite,great people...... Will deffo be there next year with me "SLIPPERY ELM" BAITS Regards,Dean
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22-01-2008, 08:05 PM
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natures catalysts
Looks like I missed out big time....havent had the chance to talk about alpha helices, Beta pleats and tertiary configurations in a long time....oh I love an enzyme or two....only problem is they dont actually work very well in your average carp bait...get denatured in the boiling process..then theres optimum temperature and pH ranges to consider!!.....
 ...nice idea but I am not convinced sure somebody can put me straight though... ....They work pretty well in the washing powders due to the temperatures and pH's and most have been modified to some extent your lipases and proteases. Heres an enzyme for you Aspartate transcarbamyolase....try saying that after a few at the show...lol.....Just waiting for a response now off carp2bait...
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Never say never
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22-01-2008, 08:06 PM
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Matt
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22-01-2008, 08:13 PM
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Hope that was 16mm barrells sunshine?????? Was nice chatting to you and your mates,look forward to speaking soon Regards,Dean
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22-01-2008, 08:40 PM
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Oh,Deano....
Some enzymes de-nature and this can be used to your advantage in disabling certain reactions You will of course,be totally familiar with the fact,that enzymes are "reaction specific",and specific enzymes break specific bonds within proteins This is extremely usefull within carp baits as once you know the specific reaction an enzyme catalyses,this then becomes rather handy...... You do not have to resort to Thermalysin (biological washing powder) to achieve anything my friend There are 1000"s of enzymes,each with a specific function,It is just the case of knowing which one,or ones to use that is the key??????? Now, if you really want to go DEEP on this one,i will bring Pete along to muddle your brain,(sorry chaps,it really was a great nite) Just waiting for a response from Deano ?????????????????????????????????????????????????? ???? lol Regards,Dean
Last edited by carp2bait@yahoo.co.uk; 22-01-2008 at 08:50 PM.
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22-01-2008, 08:53 PM
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I dont mind a good deep discussion might blow some cob webs off my cerebellum... Its an interesting topic that I have thought about a few times but having studied many enzymes and enzymatic reactions in some detail I am a little sceptical as to the merits of enzymes being incorporated into a boiled carp bait.It would be pretty easy to assess the levels of working enzymes left after the boiling process i should imagine just some simple assays using suitable substrates would be all that is required in the correct pH range.You are obviously aware that enzymes are ubiquitous in the natural world and live in the most extreme environments and can perform at almost any temperature but are you using these enzymes in your baits??Theres a well known enzyme called taq polymerase short for thermophilus aquaticus thats used to synthesise DNA that works at very high temperature as the bacteria is found in hot springs.Maybe using enzymes derived from such microbes would be useful then again maybe not as its working range is too high however it may just work its magic during the boiling process I am not saying enzymes in carp baits are 100% useless as not all will be lost during boiling but I would imagine their actual activity while on the lake bed will possibly be negligable unless you can show me evidence to the contrary. I await your response
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Never say never
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22-01-2008, 09:10 PM
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Were you at the show my friend and ventured to our little stand (which i must say looked rather buff) you would have seen our enzyme boilie in 2 different stages.....
One was a mere 8 hrs old,the other was at its 3 day stage which exhibited an exstensively softer interior to that of its younger,fresher brother...... Now,apologies to all you chaps that thought this was the "manchester carp night verdict"thread,but Deano wants it now to be called "Enzymes and their effectiveness in carp baits" thread???? Deano,it works mate and i am not the man to convince you otherwise my friend,ask those that have used it or those that have tested it,or better still let Pete and yourself start a new thread in the baits section and put this where it belongs Regards,Dean
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22-01-2008, 09:20 PM
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Sorry for the thread Hijack guys.. .but I just saw something that stimulated my brain..its been a while.....probably about as long as it takes for these enzymes to work in situ hey!!???.I would just like to know the science behind your enzyme bait and whether it will stand up to some serious scrutiny..I think I might just start a new thread in the bait section .You see it all depends on the source of your enzymes and whether maybe they have been tweaked which I very much doubt as its only the real big guns in the biotechnology field that have the science and money to do this.As you quite rightly say theres a plethora of enzymes about which will act upon any given conceivable substrate. Joe Public might just look at your baits and think hhhmmmm..enzymes must be good its sounds scientific. Without the hard science I remain sceptical. We'll switch to the right section now hey....until next time... Oh just one last retort the break down you say which is exhibited due to specific enzymatic degradation also occurs in my non enzyme impregnated baits as it does in most boiled baits after time which incidentally I believe is the prime source of attraction to mr carp and friends.Anything immersed in water is going to secrete aminos, lipids and such like after time and in turn attract fish.In fact why bother with adding expensive enzymes to your bait when the bacterial enzymes already present in the water will do this for you, food for thought maybe...
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Never say never Last edited by Deano; 22-01-2008 at 09:25 PM.
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22-01-2008, 09:31 PM
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Pete will be logging in very shortly Deano and i feel this will be an interesting thread
I wish i could be more helpfull mate but alas,i am a mere novice at this enzyme game but my partner,having had an extensive reign in a rather large pharmacutical company and whom has a rather higher IQ than myself,just may be able to satisfy your curiosity my friend,all the best matey Regards,Dean
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22-01-2008, 09:38 PM
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Its a subject which is pretty close to my heart as I spent a long time studying in this field which I thoroughly enjoyed.I am not trying to catch you out or make you look stupid although I am enjoying myself... I need hard facts you see and not pseudo science to become a convert...if it works out in your favour I'll have 10k next week!!!
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Never say never
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22-01-2008, 09:47 PM
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Regards,Dean p.s You know you want some!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! lol
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